Is there any reason you want to use ISO files?
Most people would use multi-part zip or rar files to backup the large file.
ISO is better suited to representing multiple files or an actual file system (like a representation of all files on a CD).
If you are just wanting to split and join large files, there are standard tools that are widely used such as hjsplit http://www.freebyte.com/hjsplit/
or i can just use the iso recorder to copy all of them on a CD then read the original file after booting into the CD?
If the files are small enough to fit on a single CD then you can just copy the files to the CD. If the files are larger than the capacity of a CD (or DVD) then you will need to split and/or compress the files - and will need to reconstruct the files before being able to access the files again.